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Hydrogen peroxide localization in experimental optic neuritis.

J Guy¹, E A Ellis, N A Rao

¹Department of Ophthalmology, University of Florida, College of Medicine, Gainesville 32610.

Archives of Ophthalmology (Chicago, Ill. : 1960)

|November 1, 1990

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Reactive oxygen species, specifically hydrogen peroxide (H2O2), are linked to blood-brain barrier changes in experimental optic neuritis. This study localized H2O2 to key areas, suggesting its role in disease pathogenesis.

Area of Science:

Neuroscience
Pathology
Biochemistry

Background:

Acute experimental encephalomyelitis involves blood-brain barrier (BBB) disruption.
Reactive oxygen species (ROS) are implicated in BBB alterations.
Hydrogen peroxide (H2O2) is a key ROS.

Purpose of the Study:

To investigate the association of H2O2 with altered BBB permeability in experimental optic neuritis.
To ultrastructurally localize H2O2 in the optic nerve.

Main Methods:

Modified cerium method for ultrastructural cytochemical localization of H2O2.
Examination of optic nerve tissues (lamina retinalis, choroidalis, scleralis, optic nerve head, retrobulbar nerve).
Correlation with horseradish peroxidase extravasation data.

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Main Results:

H2O2 reaction product localized to perivascular spaces and intravascular areas in the optic nerve.
H2O2 was found near intravascular leukocytes and inflammatory cells.
Extracellular H2O2 observed adjacent to axons and glial cells.

Conclusions:

H2O2 is present at sites of vascular permeability in experimental optic neuritis.
These findings support a role for H2O2 in the pathogenesis of BBB alterations.
This supports previous work on H2O2 detoxification reducing BBB permeability.