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Related Concept Videos

mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
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Related Experiment Video

Updated: May 23, 2026

Mouse In Vivo Placental Targeted CRISPR Manipulation
07:39

Mouse In Vivo Placental Targeted CRISPR Manipulation

Published on: April 14, 2023

Transcriptome landscape of the human placenta.

Jinsil Kim1, Keyan Zhao, Peng Jiang

  • 1Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA52242, USA.

BMC Genomics
|March 28, 2012
PubMed
Summary

This study reveals unique gene expression and splicing patterns in human placenta tissues. These findings offer a valuable resource for investigating placental diseases and pregnancy complications.

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Published on: August 31, 2016

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Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
08:26

Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants

Published on: August 31, 2016

Area of Science:

  • Genomics
  • Reproductive Biology
  • Molecular Biology

Background:

  • The placenta is crucial for understanding pregnancy physiology and disease.
  • Characterizing placental genes aids in identifying mechanisms of normal and abnormal pregnancies.
  • Placental tissue transcriptomes offer a resource for genomic studies of placental disease.

Purpose of the Study:

  • To deeply characterize the transcriptome of human placental tissues.
  • To compare placental gene expression and splicing with other human tissues.
  • To identify novel transcripts and exons and their role in placental function and disease.

Main Methods:

  • Deep RNA sequencing (RNA-Seq) was performed on three human placental compartments (amnion, chorion, decidua).
  • Placental RNA-Seq data were compared to 16 other human tissues.
  • Exon-level analysis and RT-PCR were used to investigate differential splicing.

Main Results:

  • Significant transcriptome differences were observed between placental compartments and other human tissues.
  • Extensive differential splicing events were identified, with 79% validated by RT-PCR.
  • The splicing regulator ESRP1 showed high expression in amnion, suggesting a role in placental-specific splicing.
  • Genes with altered expression or splicing are enriched for those implicated in placental abnormalities and preterm birth.
  • Numerous novel transcripts and exons were identified in placental tissues.

Conclusions:

  • Unique gene expression and splicing patterns in placental tissues were demonstrated.
  • These findings provide a foundation for investigating diseases related to disrupted placental gene regulation.
  • The generated data are publicly available, serving as a rich resource for placental research.