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Related Concept Videos

IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the C=O, C=N, and C=C occur between 1600–1850 cm−1.
The...
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...

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High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
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Infrared microspectroscopy using prism-based spectrographs and focal plane array detection.

Adam Lanzarotta1, Curtis Marcott, Gloria M Story

  • 1Molecular Microspectroscopy Laboratory, Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio 45056, USA.

Applied Spectroscopy
|March 28, 2012
PubMed
Summary
This summary is machine-generated.

This study integrates infrared microscopes with prism spectrographs, detailing their spatial and spectral resolution limits. The research presents experimental data on spectral resolution, range, and noise for this combined analytical system.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Microscopy

Background:

  • Infrared microscopy and prism-based spectrographs are powerful analytical tools.
  • Combining these techniques can enhance analytical capabilities.
  • Mercury cadmium telluride (MCT) detectors offer high sensitivity for infrared detection.

Purpose of the Study:

  • To integrate prism-based spectrographs with an infrared microscope.
  • To determine the spatial and spectral resolution limits of the combined system.
  • To evaluate the experimental performance and dynamic capabilities of the integrated system.

Main Methods:

  • Interfacing prism spectrographs with an infrared microscope.
  • Utilizing the microscope's aperture as the spectrograph's entrance slit.
  • Analyzing diffraction limits for both optical components.

Main Results:

  • Experimental data on spectral resolution, spectral range, and peak-to-peak noise were obtained.
  • The spatial and spectral resolution of the combined system were determined.
  • Dynamic capabilities of a specific spectrograph/microscope combination were investigated.

Conclusions:

  • The integrated spectrograph/microscope system offers valuable analytical performance.
  • Understanding diffraction limits is crucial for optimizing system resolution.
  • Further investigation into dynamic capabilities is warranted.