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Related Concept Videos

RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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Complementary DNA01:44

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Related Experiment Video

Updated: May 23, 2026

Functional Cloning Using a Xenopus Oocyte Expression System
09:40

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

Functional cDNA expression cloning: pushing it to the limit.

Hiroto Okayama1

  • 1Department of Biochemistry and Molecular Biology, Graduate School and Faculty of Medicine, The University of Tokyo, Tokyo, Japan. okayama@m.u-tokyo.ac.jp

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
|March 28, 2012
PubMed
Summary
This summary is machine-generated.

Recombinant DNA technology revolutionized life sciences. A novel gene-cloning technique, developed in the early 1980s, enables functional expression cloning of complementary DNAs (cDNAs) for molecular understanding.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Recombinant DNA technologies emerged in the 1970s-1980s, transforming life sciences.
  • Understanding gene and protein functions is crucial for explaining biological phenomena.

Purpose of the Study:

  • To review the development of a functional expression cloning technique for complementary DNAs (cDNAs).
  • To discuss the applications and future implications of this gene-cloning method in life sciences.

Main Methods:

  • Development of a novel technique for cloning full-length complementary DNAs (cDNAs).
  • Method based on functional expression in a target cell.
  • Review of historical development and applications.

Main Results:

  • The technique enables the cloning of full-length cDNAs based on their functional expression.
  • Provided tools for understanding gene and genome structures and functions at molecular levels.

Conclusions:

  • The developed gene-cloning technique significantly advanced molecular and submolecular level understanding of gene functions.
  • This method has broad implications for future life science research and applications.