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Updated: May 23, 2026
09:20
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
Published on: September 2, 2013
Tissue sample preparation in bioanalytical assays.
1Department of Chemistry, Cleveland State University, Cleveland, OH 44115, USA. kerrismith01@gmail.com
Bioanalysis
|March 29, 2012
Summary
Accurate compound quantitation in biological tissues requires robust analytical method validation. This review details tissue sample preparation, calibrator/QC preparation, and strategies to overcome common method development challenges.
Area of Science:
- Analytical Chemistry
- Biomedical Science
- Pharmacokinetics
Background:
- Analytical quantitation of compounds in biological tissues is increasingly important.
- Method validation is crucial for reliable results in tissue analysis.
- Challenges exist in sample preparation, calibrator, and quality control (QC) preparation.
Purpose of the Study:
- To review current trends in tissue sample preparation for analytical quantitation.
- To discuss considerations for calibrator and QC preparation.
- To present strategies for resolving common pitfalls in analytical method development for tissue samples.
Main Methods:
- Review of current literature on tissue sample preparation techniques.
- Discussion of homogenization, analyte fortification, and extraction methods.
- Analysis of challenges in calibrator and QC preparation for endogenous compounds.
Main Results:
- Highlights trends in tissue harvesting and homogenization.
- Addresses concerns regarding calibrator and QC preparation.
- Identifies strategies for managing stability issues and control biomatrices.
Conclusions:
- Proper analytical method validation is essential for accurate tissue compound quantitation.
- Careful design of sample preparation steps is key to successful analysis.
- Addressing potential pitfalls ensures reliable results in endogenous analysis.
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