Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026
Same author

USP24 is a cross-reactive DUB targeting MOV10 to regulate IFN-I production.

Nature communications·2026
Same author

Synthesis of Macrocyclic Peptides via Photochemical Radical Thiol-yne Reaction.

Journal of the American Chemical Society·2026
Same author

Multidimensional proteomics and explainable AI feature selection identify cross-platform lung cancer molecular signature in blood plasma.

Communications medicine·2026
Same author

Uridine cytidine kinases govern molnupiravir bioactivation and anti-SARS-CoV-2 activity.

PLoS pathogens·2026
Same author

Urine Recirculation During Normothermic Kidney Preservation Improves Energy Balance Involving the Urea and Citric Acid Cycles.

Transplantation direct·2026

Related Experiment Video

Updated: May 23, 2026

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10:25

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

Fluorescence-based active site probes for profiling deubiquitinating enzymes.

Joanna F McGouran1, Holger B Kramer, Mukram M Mackeen

  • 1Nuffield Department of Medicine, University of Oxford, Henry Welcome Building for Molecular Physiology, Roosevelt Drive, Oxford, OX3 7BN, UK.

Organic & Biomolecular Chemistry
|March 29, 2012
PubMed
Summary

Researchers developed novel ubiquitin probes with fluorescent tags to detect deubiquitinating enzyme (DUB) activity. These probes enable direct visualization of DUBs in cell extracts using in-gel fluorescence imaging.

More Related Videos

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
09:45

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay
07:05

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay

Published on: September 27, 2024

Related Experiment Videos

Last Updated: May 23, 2026

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10:25

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
09:45

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay
07:05

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay

Published on: September 27, 2024

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Molecular Biology

Background:

  • Deubiquitinating enzymes (DUBs) are crucial regulators of cellular processes.
  • Developing tools to study DUB activity is essential for understanding their roles in health and disease.

Purpose of the Study:

  • To develop and evaluate novel ubiquitin-based active site probes for detecting deubiquitinating enzyme (DUB) activity.
  • To enable direct detection and visualization of endogenous DUB activities in biological samples.

Main Methods:

  • Synthesis of novel ubiquitin-based probes incorporating a functionalizable electrophilic trap.
  • Late-stage diversification of probes for attachment of fluorescent dyes.
  • Application of probes for in-gel fluorescence imaging of DUB activity in cell extracts.

Main Results:

  • Successful development and evaluation of novel ubiquitin-based active site probes.
  • Demonstration of late-stage probe diversification via the electrophilic trap.
  • Direct detection of endogenous DUB activities in cell extracts using fluorescently labeled probes and in-gel fluorescence imaging.

Conclusions:

  • Novel ubiquitin probes with fluorescent tags are effective tools for studying DUB activity.
  • The developed probes facilitate direct visualization of endogenous DUBs, aiding in the study of their biological functions.
  • This approach offers a valuable method for investigating DUBs in complex biological systems.