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Updated: May 23, 2026

Development of a Negative Selectable Marker for Entamoeba histolytica
Published on: December 12, 2010
Purification and cellular localization of the Entamoeba histolytica transcarboxylase
E Barbosa-Cabrera1, A Salas-Casas, S Rojas-Hernández
1Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, CP 11340, Mexico, D.F., Mexico.
Abstract:
Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation.
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