Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 23, 2026

Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer
07:07

Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer

Published on: March 16, 2012

Creating transient cell membrane pores using a standard inkjet printer.

Alexander B Owczarczak1, Stephen O Shuford, Scott T Wood

  • 1Department of Bioengineering, Clemson University, USA.

Journal of Visualized Experiments : Jove
|March 29, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surrogate virus neutralization test to determine salivary neutralizing antibody inhibition of ACE2 and SARS-CoV-2 spike RBD complex.

Frontiers in immunology·2026
Same author

Comparison of slow-paced breathing interventions with and without an inhalation-hold on physiological outcomes: A randomized cross-over pilot study.

International journal of psychophysiology : official journal of the International Organization of Psychophysiology·2026
Same author

Cellular Metabolic Signatures of Long COVID-19.

Infectious disease reports·2026
Same author

Label-free, non-optical detection of intact SARS-CoV-2 virions in native human saliva via microscale surface ion conduction.

Biosensors & bioelectronics·2026
Same author

Long COVID-19: A Concept Analysis.

Infectious disease reports·2025
Same author

Microfluidic chip-based co-culture system for modeling human joint inflammation in osteoarthritis research.

Frontiers in pharmacology·2025
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Thermal inkjet bioprinting offers a fast, cell-friendly method for delivering molecules like fluorescent actin into cells. This technique creates temporary pores, enabling efficient intracellular incorporation for advanced cell dynamics studies.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioengineering

Background:

  • Bioprinting, including thermal inkjet printing, has diverse applications in tissue engineering and biosensor fabrication.
  • Thermal inkjet printing can facilitate gene transfection by temporarily disrupting cell membranes without compromising cell viability.

Purpose of the Study:

  • To demonstrate the use of thermal inkjet printing for incorporating fluorescently labeled g-actin monomers into cells.
  • To evaluate the efficiency and cell viability of thermal inkjet bioprinting for molecular delivery.

Main Methods:

  • A standard HP DeskJet 500 printer was modified for bioprinting.
  • 3T3 fibroblasts were mixed with fluorescently labeled g-actin monomers and printed onto coverslips.
  • Live cells were imaged using fluorescence microscopy.

More Related Videos

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting
05:32

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

Published on: April 21, 2021

Related Experiment Videos

Last Updated: May 23, 2026

Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer
07:07

Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer

Published on: March 16, 2012

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting
05:32

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

Published on: April 21, 2021

Main Results:

  • Thermal inkjet printing successfully introduced fluorescent actin into the cytoplasm of cells.
  • Cell viability remained comparable to standard cell plating methods.
  • The technique processed thousands of cells rapidly, with pores closing within two hours.

Conclusions:

  • Thermal inkjet bioprinting is a viable, rapid, and minimally invasive method for intracellular delivery of small molecules like proteins.
  • This technique enables the study of short-term cytoskeletal dynamics and has broad applications in cell biology.