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Simple Elimination of Background Fluorescence in Formalin-Fixed Human Brain Tissue for Immunofluorescence Microscopy
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Treatment of Schamberg's disease with advanced fluorescence technology

Danielle A Manolakos1, Jonathan Weiss, Brad Glick

  • 1Wellington Regional Medical Center/Lake Erie College of Osteopathic Medicine, Wellington, FL, USA.

Journal of Drugs in Dermatology : JDD
|March 29, 2012
PubMed

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.