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Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood
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Genetically modified pigs for biomedical research.

Yonglun Luo1, Lin Lin, Lars Bolund

  • 1Department of Biomedicine, Aarhus University, Aarhus C, Denmark. alun@hum-gen.au.dk

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Genetically modified pigs are crucial large animal models for biomedical research, aiding xenotransplantation and studying human diseases. Advances in genetic engineering and cloning have significantly expanded their utility over the past two decades.

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Area of Science:

  • Biomedical Research
  • Animal Models
  • Genetic Engineering

Background:

  • Pigs have emerged as vital large animal models in biomedical research over the last 20 years.
  • Progress in pig genomics, genetic modification, and cloning has enabled the creation of genetically modified (GM) pigs.
  • These GM pigs are instrumental for xenotransplantation and modeling human diseases.

Purpose of the Study:

  • To review key technologies for generating GM pigs.
  • To discuss genetic engineering tools used in porcine cells.
  • To summarize GM pig models developed for xenotransplantation and various human diseases.

Main Methods:

  • Review of established technologies: pronuclear microinjection, sperm-mediated gene transfer, traditional somatic cell nuclear transfer (SCNT), and handmade cloning SCNT.
  • Discussion of genetic engineering tools applicable to porcine cells.
  • Compilation of existing GM pig models and their applications.

Main Results:

  • Key technologies for generating GM pigs have been identified and summarized.
  • Various genetic engineering tools for porcine cells are outlined.
  • Numerous GM pig models have been created for xenotransplantation and modeling diseases like neurodegenerative disorders, cardiovascular diseases, cancers, diabetes, and cystic fibrosis.

Conclusions:

  • GM pig research has advanced significantly over the past two decades.
  • The development of GM pigs has greatly benefited biomedical research, particularly in xenotransplantation and disease modeling.
  • Future development in GM pig research holds promise for further breakthroughs.