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Related Experiment Video

Updated: May 23, 2026

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Methods to detect Ca(2+) in living cells.

Joseph D Bruton1, Arthur J Cheng, Håkan Westerblad

  • 1Department of Physiology & Pharmacology, Karolinska Institutet, 171 77 Stockholm, Sweden. Joseph.Bruton@ki.se

Advances in Experimental Medicine and Biology
|March 29, 2012
PubMed
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Measuring intracellular calcium ([Ca2+]i) is easier with new indicators. This chapter reviews ideal Ca2+ indicator properties, compares available options, and discusses microscopy and calibration methods for accurate measurements.

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Physiology

Background:

  • Accurate measurement of intracellular calcium concentration ([Ca2+]i) is crucial for understanding cellular processes.
  • Advancements in membrane-permeant Ca2+ indicators have simplified routine measurements.

Purpose of the Study:

  • To identify properties of an ideal Ca2+ indicator.
  • To compare available Ca2+ indicators, highlighting their advantages and disadvantages.
  • To discuss methodologies and potential errors in Ca2+ measurements.

Main Methods:

  • Review of Ca2+ indicator properties and types.
  • Discussion of epifluorescence and confocal microscopy techniques.
  • Analysis of calibration procedures for fluorescence-based Ca2+ measurements.

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Imaging Local Ca2+ Signals in Cultured Mammalian Cells
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Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Related Experiment Videos

Last Updated: May 23, 2026

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
07:17

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
11:33

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Published on: March 22, 2019

Imaging Local Ca2+ Signals in Cultured Mammalian Cells
09:30

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Main Results:

  • Ratiometric Ca2+ indicators offer advantages over non-ratiometric ones.
  • Specific equipment like microscopes is necessary for Ca2+ indicator excitation and detection.
  • Calibration is essential but challenging, with identified sources of error.

Conclusions:

  • The chapter provides a comprehensive overview of Ca2+ indicator usage.
  • It emphasizes the importance of proper methodology and calibration for accurate [Ca2+]i determination.
  • Future directions in Ca2+ detection technology are also considered.