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Related Experiment Video

Updated: May 23, 2026

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Published on: February 28, 2025

Antisense-mediated exon-skipping to induce gene knockdown.

Petra Disterer¹, Bernard Khoo

¹Department of Endocrinology, UCL Medical School, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|March 29, 2012

Summary

Antisense oligonucleotides (ASOs) offer a novel therapeutic strategy for gene silencing by inducing exon-skipping. This chapter details ASO applications and provides protocols for in vitro gene knockdown and quantification.

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Evaluation of Exon Inclusion Induced by Splice Switching Antisense Oligonucleotides in SMA Patient Fibroblasts

Evaluation of Exon Inclusion Induced by Splice Switching Antisense Oligonucleotides in SMA Patient Fibroblasts

Published on: May 11, 2018

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Antisense oligonucleotides (ASOs) are short nucleic acid sequences designed to modulate gene expression.
Exon-skipping is a technique utilizing ASOs to exclude specific exons during RNA splicing, altering protein production.
This approach holds promise for targeting disease-causing genes or specific isoforms.

Purpose of the Study:

To explore the therapeutic potential of exon-skipping antisense oligonucleotides (ASOs).
To provide a practical guide for implementing exon-skipping technology.
To demonstrate the induction and quantification of ASO-mediated exon-skipping.

Main Methods:

Utilizing exon-skipping antisense oligonucleotides (ASOs) to target specific gene sequences.
Developing an in vitro protocol for inducing exon-skipping in Apolipoprotein B (ApoB).
Employing real-time PCR for accurate quantification of exon-skipping events.

Main Results:

Successful induction of exon-skipping in Apolipoprotein B (ApoB) was achieved in vitro.
Real-time PCR provided a reliable method for quantifying the extent of exon-skipping.
The study establishes a framework for applying ASO technology therapeutically.

Conclusions:

Exon-skipping ASOs represent a viable strategy for gene expression modulation.
The provided protocols facilitate the practical application and validation of this technique.
This method has significant implications for developing targeted gene therapies.