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The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a
F Gruber1, J Visser, C P Kubicek
1Abt. Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Austria.
Abstract:
Six uridine auxotroph mutants of Trichoderma reesei QM 9414 were isolated by resistance to 5-fluoroorotic acid and one strain was identified as OMP-decarboxylase negative (pyr-) by a radiometric enzyme assay. Transformation to uridine prototrophy was achieved with the pyr4 gene of Neurospora crassa (up to 1500 transformants/micrograms) and with pyrA of Aspergillus niger (700-800 transformants/micrograms). In many transformants the PYR+ function seems to be present as extrachromosomal DNA. There is evidence for a correlation between the stability of transformants and integration of the vector in the genome whereas unstable transformants are obtained when autonomous replication of the plasmid occurs.