Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Regulation of Metabolism01:19

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epigenetic regulation by oleacein mitigates IL-1β-induced inflammation in human SW982 synovial cells.

Food & function·2026
Same author

Association between anticholinergic activity and xerostomia and/ or xerophthalmia in the elderly: systematic review.

BMC pharmacology & toxicology·2022
Same author

Efficacy of early use of remdesivir: a systematic review of subgroup analysis.

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2022
Same author

Impact of a multidisciplinary medication reconciliation program on clinical outcomes: A pre-post intervention study in surgical patients.

Research in social & administrative pharmacy : RSAP·2020
Same author

Dietary oleuropein and its acyl derivative ameliorate inflammatory response in peritoneal macrophages from pristane-induced SLE mice via canonical and noncanonical NLRP3 inflammasomes pathway.

Food & function·2020
Same author

Impact of medication reconciliation on health outcomes: An overview of systematic reviews.

Research in social & administrative pharmacy : RSAP·2019
Same journal

Knockdown of circFGFR2 inhibits prostate cancer cell metastasis and proliferation by targeting miR-221-5p/ SMUG1 pathway.....

Current medicinal chemistry·2026
Same journal

LncRNA signature associated with amino acid metabolism: A novel prognostic tool for Clear Cell Renal Cell Carcinoma.

Current medicinal chemistry·2026
Same journal

HRI Kinase Modulation by BTdCPU as a Therapeutic Strategy for Bortezomib Resistance in Prostate Cancer.

Current medicinal chemistry·2026
Same journal

EGFR Dysregulation in Cancer: From Molecular Mechanisms and Key Mutations to Evolving TKI Strategies and Resistance Mitigation.

Current medicinal chemistry·2026
Same journal

DHRS2 as a Novel Thalidomide Target Regulating Mitophagy and Inflammation in Head and Neck Squamous Cell Carcinoma.

Current medicinal chemistry·2026
Same journal

Synthetic AtMP2 from Anabas testudineus: Comprehensive ADMET and In Vivo Toxicity Assessment to Enable Future Therapeutic Development.

Current medicinal chemistry·2026
See all related articles

Related Experiment Video

Updated: May 23, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

Sirtuin modulators: mechanisms and potential clinical implications.

S Sanchez-Fidalgo1, I Villegas, M Sanchez-Hidalgo

  • 1Department of Pharmacology, School of Pharmacy, University of Seville, Spain. fidalgo@us.es

Current Medicinal Chemistry
|March 30, 2012
PubMed
Summary
This summary is machine-generated.

Sirtuins (SIRTs) are key to longevity and aging. These enzymes may protect against age-related diseases, offering potential therapeutic targets for conditions like cardiovascular disease and neurodegeneration.

Related Experiment Videos

Last Updated: May 23, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Gerontology

Background:

  • Caloric restriction and Silent Information Regulator 2 (SIR2) gene activity are linked to reduced aging damage.
  • Sirtuins (SIRTs), evolutionarily conserved proteins, are crucial for health and survival.
  • Seven mammalian SIRT classes exist, with many functions still under investigation, but they show potential in combating age-related diseases.

Purpose of the Study:

  • To review the protective roles of sirtuins against aging-associated diseases.
  • To summarize novel mechanisms underlying sirtuin activity.
  • To evaluate the potential clinical applications of sirtuin modulation.

Main Methods:

  • Review of existing literature on sirtuins and aging.
  • Analysis of studies involving molecule activators/inhibitors and genetically engineered animals.
  • Examination of sirtuin's enzymatic activity involving NAD(+) and ADP-ribosyltransferase functions.

Main Results:

  • Sirtuins are NAD(+)-dependent protein deacetylases and ADP-ribosyltransferases.
  • SIRT enzymatic activity is crucial for metabolic homeostasis, impacting tumorigenesis, inflammation, cardiovascular health, and neurodegeneration.
  • Sirtuins are implicated as key mediators of longevity across various model organisms.

Conclusions:

  • Sirtuins play a significant role in mediating the aging process.
  • Modulating sirtuin activity presents promising therapeutic avenues for age-related diseases.
  • Further research into sirtuins could lead to novel treatments for conditions like cancer, cardiovascular diseases, and neurodegenerative disorders.