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Anti-H-2 antibodies induced by syngeneic immunization
P Iványi1, P van Mourik, M Breuning
1Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, University of Amsterdam, Amsterdam, The Netherlands.
Abstract:
Abstract. Alloreactive cytotoxic antibodies were induced in BALB/c mice by syngeneic immunization with normal lymphoid cells. Sixteen out of 41 mice produced antibodies with distinct anti-H-2 specificity. Anti-Kk antibodies were present in all positive sera, but the individual sera produced different reactivity patterns when tested on a panel of H-2 haplotypes. Absorption and immunoprecipitation experiments confirmed the H-2 specificity of the syngeneic sera. We hypothesize that virus-modified H-2d structures have triggered alloreactive B-cell clones to produce anti-H-2 antibodies.
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