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Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Published on: June 23, 2023

Genomic insights into bacterial DMSP transformations.

Mary Ann Moran¹, Chris R Reisch, Ronald P Kiene

¹Department of Marine Sciences, University of Georgia, Athens, Georgia 30602, USA. mmoran@uga.edu

Annual Review of Marine Science

|March 31, 2012

Summary

This summary is machine-generated.

Scientists identified key genes for bacterial dimethylsulfoniopropionate (DMSP) breakdown in oceans. These pathways influence marine food webs and atmospheric sulfur, with 60% of surface bacteria capable of DMSP degradation.

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Area of Science:

Marine microbiology
Biogeochemical cycles
Genomics

Background:

Bacterial degradation of dimethylsulfoniopropionate (DMSP) is crucial for marine ecosystems.
Understanding the genetic basis of DMSP breakdown pathways is essential for predicting its impact on ocean-atmosphere sulfur exchange.
Previous research has advanced the understanding of DMSP metabolism, but regulatory factors remain unclear.

Purpose of the Study:

To identify and characterize the genes involved in the two primary bacterial DMSP degradation pathways.
To assess the potential contribution of marine bacteria to DMSP processing based on gene abundance.
To investigate the regulation of competing DMSP degradation pathways in natural bacterioplankton communities.

Main Methods:

Genomic and functional genomic analyses were employed.
Gene identification for DMSP demethylation (dmdA-D) and cleavage (dddD-Y) pathways.
Ocean metagenomic data analysis to quantify gene prevalence.
Gene transcription analyses of natural bacterioplankton communities.

Main Results:

Two main bacterial DMSP degradation pathways were genetically defined: demethylation (dmd genes) and cleavage (ddd genes).
The dmd pathway retains carbon and sulfur in the marine food web.
The ddd pathway produces dimethylsulfide (DMS), impacting ocean-atmosphere sulfur flux.
Genes for DMSP degradation are present in 60% of surface ocean bacterial cells.
Factors regulating these competing pathways are still under investigation.

Conclusions:

The identification of key DMSP degradation genes provides a foundation for understanding bacterial roles in marine sulfur cycling.
The prevalence of these genes suggests widespread bacterial capacity for DMSP processing in surface oceans.
Further research into gene transcription is needed to elucidate the regulation of DMSP pathways and their ecological significance.