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Related Concept Videos

Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction
05:33

Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction

Published on: July 26, 2022

Exploring the environmental transmission electron microscope.

Jakob B Wagner1, Filippo Cavalca, Christian D Damsgaard

  • 1Center for Electron Nanoscopy, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. jakob.wagner@cen.dtu.dk

Micron (Oxford, England : 1993)
|March 31, 2012
PubMed
Summary
This summary is machine-generated.

Environmental transmission electron microscopy (ETEM) enables high-resolution, in situ material analysis under realistic conditions. Combining ETEM with other techniques offers new insights into material behavior in various gaseous environments.

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Physics

Background:

  • In situ techniques are advancing, making realistic condition measurements feasible for transmission electron microscopy (TEM).
  • Environmental TEM (ETEM) allows high spatial resolution analysis under controlled environments.

Purpose of the Study:

  • To explore opportunities offered by combining environmental TEM (ETEM) with other in situ techniques.
  • To investigate the use of adjacent setups with advanced transfer methods for in situ measurements.
  • To demonstrate these techniques using Au/MgO and Cu(2)O material systems in gaseous environments.

Main Methods:

  • Utilizing environmental transmission electron microscopy (ETEM) for in situ analysis.
  • Combining electron-based and photon-based techniques within the microscope.
  • Employing sophisticated transfer methods for specialized in situ equipment.

Main Results:

  • Demonstrated feasibility of high-resolution in situ measurements under near-realistic conditions using ETEM.
  • Illustrated combined electron and photon techniques for enhanced material analysis.
  • Showcased successful application on Au/MgO and Cu(2)O systems in diverse gaseous environments.

Conclusions:

  • Environmental TEM, especially when combined with other in situ methods, significantly expands analytical capabilities.
  • Advanced transfer systems maintain the integrity of in situ measurements.
  • These integrated approaches provide valuable insights into material behavior under operational conditions.