Related Experiment Video
Updated: May 23, 2026
Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration
Published on: June 7, 2012
Effect of ovariectomy on contrast agent diffusion into lumbar intervertebral disc: a dynamic contrast-enhanced MRI
Min Deng1, James F Griffith, Xiao-Ming Zhu
1Department of Imaging and Interventional Radiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, People's Republic of China.
Purpose:
The purpose was to study the effect of estrogen deficiency on contrast agent diffusion into intervertebral disc in a rat model.
Materials And Methods:
Seven-month-old female Sprague-Dawley rats were used. Fourteen rats had ovariectomy, and nine rats had sham surgery. Magnetic resonance imaging (MRI) of sagittal midsection of lumbar spine was performed with a 1.5-T magnet. Dynamic MRI was performed after a bolus injection of Gd-DOTA (0.3 mmol/kg) through tail vein. Eight hundred images were acquired at 0.6 s per acquisition. Regions of interests were drawn over three discs per rat. Maximum enhancement (E(max)) and enhancement slope (E(slope)) were evaluated. MRI was carried out at baseline and 8 weeks postsurgery.
Result:
All disc enhancements demonstrated an initial fast wash-in phase followed by a second slower wash-in phase. For initial wash-in phase, E(1)(max) and E(1)(slope) of all rats remained unchanged at the two time points. For second wash-in phase, E(2)(max) and E(2)(slope) of control rats remained unchanged, while with ovariectomized rats, E(2)(max) showed reduction at 8 weeks (4.5%±5.6%) compared to baseline (10.3%±6.3%, P=.037), and E(2)(slope) was lower at 8 weeks (0.015±0.017) than the baseline (0.029±0.022), although it was not statistically significant (P=.101).
Conclusion:
Ovariectomy induced detectable decrease in second wash-in phase of contrast agent into lumbar disc.
