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Evaluation of multiplex polymerase chain reaction and microarray-based assay for rapid herpesvirus diagnostics
Laura Mannonen1, Raija Vainionpää, Juha Kauppinen
1Department of Virology, Helsinki University Central Hospital Laboratory Services (HUSLAB), Helsinki, Finland. Laura.Mannonen@hus.fi
Diagnostic Microbiology and Infectious Disease
|March 31, 2012
Summary
A new microarray assay accurately diagnoses 7 herpesviruses in central nervous system (CNS) infections. This rapid diagnostic tool improves patient care, especially for immunocompromised individuals with CNS infections.
Area of Science:
- Clinical Microbiology
- Molecular Diagnostics
- Virology
Background:
- Central nervous system (CNS) infections require rapid diagnosis to reduce severe outcomes.
- Current diagnostic methods for viral CNS infections can be time-consuming.
- Herpesviruses are significant causes of CNS infections, necessitating accurate detection.
Purpose of the Study:
- To evaluate the performance of a multiplex polymerase chain reaction (PCR) and microarray-based assay, Prove-it™ Herpes, for diagnosing 7 herpesviruses in CNS infections.
- To assess the utility of the microarray assay in a routine clinical laboratory setting.
Main Methods:
- Analysis of 495 cerebrospinal fluid (CSF) samples using both existing laboratory PCR methods and the Prove-it™ Herpes microarray assay.
- Comparison of diagnostic accuracy, including sensitivity and specificity, between the two methods.
Main Results:
- The microarray assay demonstrated high diagnostic accuracy with 93% sensitivity and 99% specificity.
- The assay proved to be a rapid and robust platform, easily integrated into laboratory workflows.
- The method requires a small sample volume and covers a broad range of herpesviruses.
Conclusions:
- The Prove-it™ Herpes microarray assay is a valuable tool for the rapid and accurate diagnosis of herpesviral CNS infections.
- Its implementation can lead to improved patient management and treatment, particularly for vulnerable populations like immunocompromised patients.
