Application of creatinine-sensitive biosensor for hemodialysis control

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Clinical Diagnostics

Background:

• Potentiometric biosensors offer sensitive and selective analyte detection.
• Previous work established a biosensor for creatinine using pH-sensitive field-effect transistors and creatinine deiminase.
• Optimizing biosensors for real-world clinical samples is crucial for practical application.

Purpose of the Study:

• To optimize a previously developed potentiometric biosensor for creatinine determination.
• To validate the biosensor's performance in real patient dialysate samples.
• To enable on-line monitoring of creatinine levels during dialysis sessions.

Main Methods:

• Utilized pH-sensitive field-effect transistors as transducers.
• Immobilized creatinine deiminase (EC 3.5.4.21) as the biorecognition element.
• Applied the optimized biosensor for on-line analysis of creatinine in patient dialysate.

Main Results:

• The optimized biosensor demonstrated high sensitivity and selectivity for creatinine.
• Successful on-line monitoring of creatinine levels in dialysate during dialysis was achieved.
• A high correlation was observed between biosensor measurements and the traditional Jaffe method.

Conclusions:

• The optimized potentiometric biosensor is effective for creatinine analysis in real dialysate samples.
• The biosensor facilitates real-time monitoring of creatinine levels in patients with renal failure.
• This technology holds promise for improved clinical diagnostics and patient management during dialysis.