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Mouse intragastric infusion (iG) model.

Akiko Ueno1, Raul Lazaro, Ping-Yen Wang

  • 1Southern California Research Center for ALPD and Cirrhosis, Keck School of Medicine of the University of Southern California, Los Angeles, California, USA.

Nature Protocols
|March 31, 2012
PubMed
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This study introduces a rodent intragastric infusion (iG) model for long-term nutrient delivery. This method offers precise control for research in gastrointestinal physiology, metabolism, and disease models.

Area of Science:

  • Gastroenterology and Hepatology
  • Pharmacology and Toxicology
  • Metabolic Research

Background:

  • Accurate nutrient and substance delivery is crucial for rodent research.
  • Existing methods may lack long-term control or flexibility.
  • Understanding gastrointestinal (GI) physiology and neuroendocrinology requires precise experimental models.

Purpose of the Study:

  • To present a reliable method for direct intragastric delivery in rodents.
  • To highlight the model's utility in various research areas, including obesity and liver disease.
  • To demonstrate the model's adaptability for studying bypassed GI segments and genetic factors.

Main Methods:

  • Chronic implantation of a gastrostomy catheter in rodents (mice and rats).
  • Utilizing a flow-through swivel system for long-term (2-3 months) infusion.

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  • Combining intragastric infusion with ad libitum solid diet feeding.

    • Main Results:

    • Achieved maximal control over the rate and pattern of substance delivery.
    • Demonstrated feasibility for long-term (2-3 months) direct intragastric infusion.
    • Showcased the model's versatility for diverse research applications.

    Conclusions:

    • The rodent intragastric infusion (iG) model provides precise, long-term delivery control.
    • This model is valuable for studying food intake, GI physiology, drug metabolism, obesity, and liver disease.
    • The model can be adapted for investigating GI neuroendocrinology and genetic factors in disease.