Related Experiment Video

Updated: May 23, 2026

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
05:57

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

Published on: May 16, 2011

Extracellular proteins from Lactobacillus plantarum BMCM12 prevent adhesion of enteropathogens to mucin

Borja Sánchez1, María C Urdaci

  • 1Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas, Ctra. Infiesto s/n, 33300 Villaviciosa, Asturias, Spain. borja@ipla.csic.es

Current Microbiology
|March 31, 2012
PubMed

Insights

Extracellular proteins from Lactobacillus plantarum BMCM12 significantly inhibit gut pathogen adhesion to mucin. These findings highlight the potential of these proteins in preventing pathogen colonization in the gut.

Area of Science:

  • Microbiology
  • Proteomics
  • Gastroenterology

Background:

  • Gut pathogens can adhere to the intestinal lining, leading to infections.
  • Lactobacillus plantarum is a probiotic bacterium with potential health benefits.
  • Extracellular proteins secreted by probiotics may play a role in modulating the gut environment.

Purpose of the Study:

  • To investigate the anti-adhesion properties of extracellular proteins from Lactobacillus plantarum BMCM12 against gut pathogens.
  • To identify and characterize these extracellular proteins.

Main Methods:

  • Precipitation and SDS-PAGE of extracellular proteins from L. plantarum BMCM12.
  • Tandem mass spectrometry for protein identification.
  • Glycoprotein staining and de-glycosylation assays.
  • In vitro assays to assess pathogen adhesion to mucin (using Escherichia coli and Salmonella enterica).

Main Results:

  • Extracellular proteins from L. plantarum BMCM12 were identified, with evidence of glycosylation.
  • These proteins significantly reduced the adhesion of Escherichia coli and Salmonella enterica to mucin.
  • The inhibitory effect was observed when proteins were added prior to pathogen exposure.

Conclusions:

  • Extracellular proteins from L. plantarum BMCM12 possess anti-adhesion properties against common gut pathogens.
  • These proteins are potential candidates for developing strategies to prevent pathogen colonization in the gut.
  • Protein glycosylation may contribute to the functional activity of these extracellular proteins.

More Related Videos

Adherence of Bacteria to Plant Surfaces Measured in the Laboratory07:07

Adherence of Bacteria to Plant Surfaces Measured in the Laboratory

Published on: June 19, 2018

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells07:21

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

Published on: September 17, 2021

Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Adherens Junctions01:24

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Surface Membrane Barriers01:18

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
Probiotics01:22

Probiotics

Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
Laminins are the Adhesive Proteins of Basal Lamina00:55

Laminins are the Adhesive Proteins of Basal Lamina

Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains.
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
Mucosal Barrier of the Stomach01:25

Mucosal Barrier of the Stomach

The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...