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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

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Multimodal Optical Imaging Platform for Studying Cellular Metabolism
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Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

Multimodal optical imaging with multiphoton microscopy and optical coherence tomography.

Shuo Tang1, Yifeng Zhou, Myeong Jin Ju

  • 1Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, Canada. tang@ece.ubc.ca

Journal of Biophotonics
|March 31, 2012
PubMed
Summary

This study compares two combined multiphoton microscopy and optical coherence tomography (MPM/OCT) systems for multimodal imaging. Single-scale MPM/OCM offers simultaneous high-resolution imaging, while multi-scale MPM/OCT provides variable fields-of-view for tissue and cellular analysis.

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Area of Science:

  • Biomedical Optics
  • Microscopy and Imaging Technologies

Background:

  • Multimodal optical imaging combines different techniques to provide complementary information.
  • Multiphoton microscopy (MPM) offers high-resolution cellular imaging with intrinsic contrast (two-photon excited fluorescence - TPEF, second harmonic generation - SHG).
  • Optical coherence tomography (OCT) provides label-free, cross-sectional imaging of tissue microstructure at varying resolutions.

Purpose of the Study:

  • To compare two configurations of combined MPM and OCT systems for advanced biological specimen imaging.
  • To evaluate the strengths and limitations of single-scale MPM/OCM versus multi-scale MPM/OCT.

Main Methods:

  • Development and comparison of two MPM/OCT systems: single-scale MPM/OCM and multi-scale MPM/OCT.
  • Both systems utilize MPM with TPEF and SHG channels.
  • Multi-scale MPM/OCT employs objectives with different numerical apertures (NA) to achieve variable fields-of-view (FOVs).

Main Results:

  • Single-scale MPM/OCM simultaneously acquires multiple contrasts with high resolution but limited FOV.
  • Multi-scale MPM/OCT enables imaging across different FOVs, with OCT for tissue-level and MPM for cellular-level detail.
  • The utility of OCT in multi-scale MPM/OCT varies depending on sample properties.

Conclusions:

  • Both single-scale MPM/OCM and multi-scale MPM/OCT systems offer distinct advantages for multimodal optical imaging.
  • Future integration of both system functionalities into a single platform is proposed for enhanced capabilities.