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Updated: May 23, 2026

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Published on: June 12, 2018
MicroRNA degradation and turnover: regulating the regulators
Zhuo Zhang1, Yong-Wen Qin, Gary Brewer
1Department of Cardiology, Changhai Hospital, Shanghai, China.
Abstract:
MicroRNAs (miRNAs) are endogenous, small noncoding RNAs that play important regulatory roles in gene expression. The control of miRNA biogenesis has been well characterized, but their degradation is not fully understood. Recent discoveries indicate that miRNAs have a long life span in general. However, rapid turnover dynamics of miRNAs in a variety of specific cellular contexts has been documented, as well as the requirement of sequence elements for miRNA decay. Furthermore, several ribonucleases that degrade miRNAs have been identified. Here, we discuss the cellular contexts and biochemical mechanisms of miRNA decay, together with several prominent questions in this field.
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