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Published on: December 10, 2016
Draft genome sequence of the nontoxigenic Clostridium difficile strain CD37
Michael S M Brouwer1, Elaine Allan, Peter Mullany
1UCL Eastman Dental Institute, London, UK.
Journal of Bacteriology
|March 31, 2012
Summary
The first nontoxigenic Clostridium difficile strain, CD37, has had its genome sequenced. This analysis revealed two mobile genetic elements, specifically putative conjugative transposons, within its DNA.
Area of Science:
- Microbiology
- Genomics
Background:
- Clostridium difficile is a significant human pathogen.
- Previous sequencing efforts have focused on toxigenic strains.
- All previously sequenced Clostridium difficile strains harbor mobile genetic elements.
Purpose of the Study:
- To report the draft genome sequence of Clostridium difficile strain CD37.
- To characterize the mobile genetic elements present in a nontoxigenic strain.
Main Methods:
- Whole-genome sequencing of Clostridium difficile strain CD37.
- Bioinformatic analysis of the resulting genome sequence.
Main Results:
- The draft genome sequence of Clostridium difficile strain CD37 was successfully generated.
- Strain CD37 was confirmed as nontoxigenic.
- Two putative conjugative transposons were identified within the CD37 genome.
Conclusions:
- The genome sequence provides a valuable resource for studying nontoxigenic Clostridium difficile.
- The presence of conjugative transposons in a nontoxigenic strain warrants further investigation into their role and transfer.
- Comparative genomic studies can benefit from the inclusion of this strain's sequence.
