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Updated: May 23, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

p53-induced gene 3 mediates cell death induced by glutathione peroxidase 3

Hui Wang1, Katherine Luo, Lang-Zhu Tan

  • 1Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA.

The Journal of Biological Chemistry
|March 31, 2012
PubMed

Insights

Glutathione peroxidase 3 (GPx3) interacts with PIG3 to promote cell death in prostate cancer. This GPx3-PIG3 signaling pathway is crucial for apoptosis, offering new therapeutic targets for cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Death Signaling

Background:

  • Glutathione peroxidase 3 (GPx3) expression is reduced in many cancers, including prostate cancer, suggesting a tumor suppressor role.
  • The precise molecular mechanisms underlying GPx3's tumor suppressive functions, particularly in prostate cancer, are not fully understood.

Purpose of the Study:

  • To elucidate the mechanism of GPx3-mediated tumor suppression in prostate cancer.
  • To investigate the interaction between GPx3 and p53-induced gene 3 (PIG3) in regulating cell death.

Main Methods:

  • Overexpression of GPx3 in prostate cancer cell lines (DU145, PC3) and immortalized prostate epithelial cells (RWPE-1).
  • Utilizing a peroxidase-negative GPx3 mutant (GPx3(x73c)) and PIG3 knockdown.
  • Assessing reactive oxygen species (ROS) levels, caspase-3 activity, and apoptotic cell death.
  • Investigating UV-induced apoptosis in RWPE-1 cells with manipulated GPx3 and PIG3 levels.

Main Results:

  • Forced GPx3 expression, including a mutant form, increased apoptosis in prostate cancer cells.
  • GPx3 overexpression led to increased reactive oxygen species and caspase-3 activity, which were dependent on PIG3.
  • GPx3 and PIG3 signaling was found to be critical for UV-induced apoptosis.

Conclusions:

  • GPx3 directly interacts with PIG3, forming a novel signaling pathway that regulates cell death in prostate cancer.
  • This GPx3-PIG3 pathway plays a significant role in apoptosis, potentially offering new therapeutic strategies for prostate cancer.

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