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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...

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Related Experiment Video

Updated: May 23, 2026

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

Mass spectrometry-based proteomics for translational research: a technical overview.

Joao A Paulo1, Vivek Kadiyala, Peter A Banks

  • 1Center for Pancreatic Disease, Division of Gastroenterology, Hepatology and Endoscopy, Brigham and Women’s Hospital and Department of Medicine, Harvard Medical School, Boston, MA, USA. joao.paulo@childrens.harvard.edu

The Yale Journal of Biology and Medicine
|March 31, 2012
PubMed
Summary

Mass spectrometry techniques identify protein biomarkers in clinical samples for high-throughput analysis. This overview covers methods, limitations, and potential for disease mechanism research.

Keywords:
biomarkerschronic pancreatitismass spectrometrypancreas

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

  • Clinical proteomics
  • Biomarker discovery
  • Mass spectrometry

Background:

  • Clinical sample analysis is crucial for identifying disease biomarkers.
  • Mass spectrometry offers high-throughput proteomic investigation capabilities.

Purpose of the Study:

  • To provide an overview of mass spectrometry-based proteomic techniques for clinical sample analysis.
  • To discuss sample handling, mass spectrometry methods, and quantitative approaches.
  • To examine the limitations and future potential of these technologies.

Main Methods:

  • Liquid chromatography fractionation coupled with tandem mass spectrometry.
  • High-throughput proteomic techniques for clinical samples.
  • Protein extraction, fractionation, and quantitative proteomics.

Main Results:

  • Mass spectrometry-based proteome elucidation can identify potential protein biomarkers.
  • Tandem mass spectrometry effectively handles complex protein mixtures.
  • This approach aids in generating hypotheses for disease mechanism studies.

Conclusions:

  • Mass spectrometry is a powerful tool for biomarker discovery in clinical settings.
  • Understanding proteomic techniques is key to advancing disease research.
  • Further development holds significant potential for clinical diagnostics and therapeutics.