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Related Experiment Videos

Muscle buffer capacity estimated from pH changes during rest-to-work transitions.

G R Adams1, J M Foley, R A Meyer

  • 1Department of Physiology, Michigan State University, East Lansing 48824.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|September 1, 1990
PubMed
Summary

During muscle contraction, phosphocreatine (PCr) hydrolysis causes an increase in intracellular pH. This study confirms that PCr breakdown accounts for the observed pH changes in skeletal muscle.

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Area of Science:

  • Physiology
  • Biochemistry
  • NMR Spectroscopy

Background:

  • Skeletal muscle contraction involves metabolic processes that can alter intracellular pH.
  • Phosphocreatine (PCr) is a key energy buffer in muscle, and its hydrolysis releases protons.
  • Understanding pH regulation during exercise is crucial for comprehending muscle function and fatigue.

Purpose of the Study:

  • To investigate the relationship between phosphocreatine (PCr) hydrolysis and intracellular pH changes in skeletal muscle during stimulation.
  • To quantify the buffering capacity of skeletal muscle using nuclear magnetic resonance (NMR) spectroscopy.
  • To determine if PCr hydrolysis alone explains the observed pH shifts in the initial seconds of muscle contraction.

Main Methods:

  • Gated phosphorus-31 nuclear magnetic resonance (31P-NMR) spectroscopy was used to monitor PCr levels and intracellular pH in rat and cat skeletal muscles.

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  • Muscles were stimulated at 5 Hz, and spectra were acquired at 5 or 9 seconds.
  • Buffer capacity (beta) was determined both in vivo from PCr and pH changes and in vitro by titrating muscle homogenates.

    • Main Results:

    • Net PCr hydrolysis was associated with intracellular alkalinization in cat biceps, cat soleus, and rat gastrocnemius muscles.
    • Apparent buffer capacity calculated from in vivo measurements closely matched predictions from in vitro homogenate titrations.
    • The observed pH changes during the initial seconds of contraction were fully explained by proton consumption from PCr hydrolysis.

    Conclusions:

    • Proton production from phosphocreatine hydrolysis directly influences intracellular pH during the early stages of skeletal muscle contraction.
    • Skeletal muscle possesses significant buffering capacity, which can be accurately estimated using NMR spectroscopy and homogenate titrations.
    • These findings provide a quantitative understanding of pH dynamics and energy metabolism in contracting muscle.