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Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: May 23, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

Dielectrophoretic cell capture on polyester membranes.

Conni Hanke, Petra S Dittrich, Darwin R Reyes

    ACS Applied Materials & Interfaces
    |April 3, 2012
    PubMed
    Summary
    This summary is machine-generated.

    A novel system enables dielectrophoretic cell capture using patterned polyester membranes. This technology facilitates cell separation and viability in microfluidic devices for cell interaction studies.

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    Area of Science:

    • Biomedical Engineering
    • Microfluidics
    • Cell Biology

    Background:

    • Developing advanced cell capture systems is crucial for biological research.
    • Existing methods often lack the precision for complex cell-based assays.
    • Microfluidic devices offer controlled environments for cell manipulation.

    Discussion:

    • This study introduces a new dielectrophoretic cell capture system.
    • Gold microelectrodes were fabricated on polyester membranes using photolithography.
    • Membrane properties (roughness, permeability, hydrophilicity) remained unchanged post-fabrication.

    Key Insights:

    • Successful demonstration of dielectrophoretic cell capture and viability on patterned membranes.
    • The fabricated microelectrodes are compatible with standard membrane materials.
    • This system enables spatial separation of different cell types in microfluidic devices.

    Outlook:

    • Potential integration with multilayer microfluidic systems.
    • Enables powerful tools for studying cell-cell interactions in co-culture.
    • Facilitates research requiring distinct microenvironments for different cell types.