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Updated: Mar 2, 2026
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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
Published on: September 26, 2025
634
CDC6 mRNA fluctuates periodically in the yeast cell cycle
1Department of Pediatrics and Microbiology, University of Southern California School of Medicine, Los Angeles 90027.
The Journal of Biological Chemistry
|November 15, 1990
Summary
The Saccharomyces cerevisiae CDC6 gene shows periodic expression during the cell cycle, peaking at the G1/S phase boundary. This regulation differs from histone H2A mRNA, suggesting distinct control mechanisms.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- Understanding cell cycle regulation is crucial for cell division and development.
- The Saccharomyces cerevisiae CDC6 gene plays a role in cell cycle progression.
- Previous studies have identified various genes involved in yeast cell cycle control.
Purpose of the Study:
- To investigate the cell cycle-specific expression pattern of the Saccharomyces cerevisiae CDC6 gene.
- To compare the regulation of CDC6 gene expression with other cell cycle-related genes like CDC8 and histone H2A.
- To identify potential regulatory elements in the CDC6 promoter region.
Main Methods:
- Synchronization of yeast cultures using alpha-factor arrest and centrifugal elutriation.
- Quantitative analysis of CDC6, CDC8, and histone H2A mRNA levels throughout the cell cycle.
- Treatment with hydroxyurea to assess the impact on mRNA stability.
- DNA sequence analysis of the CDC6 5'-flanking region.
Main Results:
- CDC6 gene expression is periodic, with transcript levels increasing in late G1 and peaking at the G1/S phase boundary.
- CDC6 and CDC8 mRNA peaks precede histone H2A mRNA peaks.
- Hydroxyurea treatment did not affect CDC6 or CDC8 mRNA levels, unlike histone H2A mRNA.
- The CDC6 promoter contains repeated sequence elements (5'-C/GACGCGNC/G-3' and 5'-PuGNAGAAA-3') also found in other cell cycle-regulated genes.
Conclusions:
- CDC6 gene expression is tightly regulated in a cell cycle-dependent manner.
- The distinct regulation of CDC6/CDC8 compared to histone H2A suggests different regulatory pathways.
- Identified promoter sequence elements may play a significant role in the cell cycle-specific transcription of CDC6 and other related genes.
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