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Updated: Feb 9, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Two-color DNA nanoprobe of intracellular dynamics
Joshua N Milstein1, Mike Chu, Krishnan Raghunathan
1Department of Chemical and Physical Sciences, University of Toronto Mississauga, Mississauga, ON L5L 1C6. josh.milstein@utoronto.ca
Abstract:
We have developed a correlation microscopy technique to follow the dynamics of quantum dot labeled DNA within living cells. The temporal correlation functions of the labels reflect the fluctuations of the DNA nanoprobe as a result of its interactions with the cellular environment. They provide a sensitive measure for the length of the probe on the scale of a persistence length (∼50 nm) and reveal strong nonthermal dynamics of the cell. These results pave the way for dynamic observations of DNA conformational changes in vivo.
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