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Related Experiment Video

Updated: May 23, 2026

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
08:28

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Published on: June 30, 2017

Adipogenesis: from stem cell to adipocyte.

Qi Qun Tang1, M Daniel Lane

  • 1Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Annual Review of Biochemistry
|April 3, 2012
PubMed
Summary

Excessive calorie intake leads to increased adipocyte number and obesity. Signaling factors and transcription factors like C/EBPβ drive mesenchymal stem cell differentiation into adipocytes, contributing to fat tissue expansion.

Area of Science:

  • Cell Biology
  • Metabolic Regulation
  • Stem Cell Biology

Background:

  • Obesity results from excessive caloric intake and energy expenditure imbalance.
  • Adipocyte hyperplasia, an increase in fat cell number, contributes to obesity.
  • Mesenchymal stem cells (MSCs) in adipose tissue are precursors to new adipocytes.

Purpose of the Study:

  • To elucidate the molecular mechanisms driving adipocyte hyperplasia.
  • To identify key signaling pathways and transcription factors involved in adipogenesis.
  • To understand how mesenchymal stem cells differentiate into mature fat cells.

Main Methods:

  • Investigated signaling factors (BMP, Wnt) mediating stem cell commitment.
  • Examined differentiation inducers (IGF1, glucocorticoid, cAMP) triggering adipocyte cell cycle reentry.

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Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
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Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

Published on: March 7, 2015

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Last Updated: May 23, 2026

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
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Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

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Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
07:35

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

Published on: March 7, 2015

  • Analyzed transcription factor cascade involving C/EBPβ, PPARγ, and C/EBPα phosphorylation and activation.

    • Main Results:

    • BMP and Wnt families are crucial for MSC commitment to preadipocytes.
    • Growth-arrested preadipocytes require specific inducers for DNA replication and mitotic clonal expansion.
    • Phosphorylation of C/EBPβ by MAP kinase and GSK3β is essential for its DNA-binding activity.
    • Activated C/EBPβ induces PPARγ and C/EBPα, initiating adipocyte gene expression.

    Conclusions:

    • Adipocyte hyperplasia is a regulated process involving stem cell differentiation and proliferation.
    • Specific signaling pathways and transcription factor activation are critical for adipogenesis.
    • Understanding these mechanisms provides insight into obesity and metabolic disease.