Related Experiment Video
Updated: May 23, 2026
Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
Published on: July 12, 2018
Improving on SUVA 254 using fluorescence-PARAFAC analysis and asymmetric flow-field flow fractionation for assessing
Ashley D Pifer1, Julian L Fairey
1Department of Civil Engineering, University of Arkansas, 4190 Bell Engineering Center, Fayetteville, AR 72701, USA.
Abstract:
Several challenges with disinfection byproduct (DBP) control stem from the complexity and diversity of dissolved organic matter (DOM), which is ubiquitous in natural waters and reacts with disinfectants to form DBPs. Fluorescence parallel factor (PARAFAC) analysis and asymmetric flow-field flow fractionation (AF4) were used in combination with free chlorine DBP formation potential (DBPFP) tests to study the physicochemical DOM properties and DBP formation in raw- and alum-coagulated waters. Enhanced coagulation with alum became more effective at removing DBP-precursors as the pH decreased from 8 to 6. AF4-UV(254) fractograms indicated enhanced coagulation at pH 6 preferentially removed larger DOM, whereas no preferential size removal occurred at pH 8. Fluorescence-PARAFAC analysis revealed the presence of one protein-like and three humic-like fluorophore groups; stronger linear correlations were found between chloroform and the maximum intensity (F(MAX)) of a humic-like fluorophore (r(2) = 0.84) than with SUVA(254) (r(2) = 0.51). This result indicated that the fluorescence-PARAFAC approach used here was an improvement on SUVA(254), i.e., fluorescence-based measurements were stronger predictors of chloroform formation.
More Related Videos
09:33Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension
Published on: September 11, 2020
12:11A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
Published on: July 9, 2012