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Highly sensitive detection of alkaline phosphatase using molecular beacon probes based on enzymatic polymerization

Changbei Ma1

  • 1School of Biological Science and Technology, Central South University, Changsha 410013, China. lhlmcb@yahoo.com.cn

Molecular and Cellular Probes
|April 3, 2012
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...