High-throughput species identification of enterococci using pyrosequencing
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Updated: May 23, 2026

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Pyrosequencing for Microbial Identification and Characterization
Published on: August 22, 2013
Rahat Zaheer1, L Jay Yanke, Deirdre Church
1Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta, Canada T1J 4B1.
Journal of Microbiological Methods
|April 3, 2012
Abstract:
Here we report the development and validation of an automated high-throughput pyrosequencing-based method for the reliable identification of isolated Enterococcus species. This method exploits the discrete species-specificity of hypervariable groES-EL spacer region and utilizes a universal dispensation order optimized for a wide range of Enterococcus species.
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