Single DNA molecule denaturation using laser-induced heating
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Updated: Apr 29, 2026

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
Published on: January 31, 2018
Min-Sheng Hung1, Osamu Kurosawa, Masao Washizu
1Department of Biomechatronic Engineering, National Chiayi University, No. 300 Syuefu Road, Chiayi 600, Taiwan. mshung@mail.ncyu.edu.tw
Abstract:
This paper proposes targeted in situ denaturation through laser-induced heating to partially amplify relevant sequences from a long DNA strand. It uses 5 kb of DNA as a sample, labeling both strands with quantum dots, with one strand immobilized on a solid surface. We irradiated a targeted DNA sequence with a focused infrared laser to elevate its temperature, monitoring the process by microscope. The denaturation was detected in real time by separating quantum dots on each strand. Results showed that complete separation of the strands occurred within a few seconds of laser irradiation, which raised the temperature to approximately 90 °C.
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