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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: May 23, 2026

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
07:42

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Published on: April 2, 2008

[Development of fluorescent labeling methods for stem cells].

Yuriko Higuchi1

  • 1Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan. higuchi@pharm.kyoto-u.ac.jp

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|April 3, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed long-term fluorescent labeling for mesenchymal stem cells (MSCs) using modified quantum dots. This enables in vivo imaging to track cell dynamics, crucial for advancing cell-based therapies.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Medical Imaging

Background:

  • Cell-based therapies require understanding transplanted cell disposition and differentiation.
  • In vivo imaging offers real-time insights into cell behavior post-transplantation.
  • Existing imaging methods like PET and MRI have limitations for single-cell visualization.

Purpose of the Study:

  • To develop a robust method for long-term fluorescent labeling of mesenchymal stem cells (MSCs).
  • To enable real-time in vivo imaging of MSCs dynamics after transplantation.
  • To assess the utility of PAMAM dendrimer-modified quantum dots for cell tracking.

Main Methods:

  • Developed polyamidoamine (PAMAM) dendrimer-modified quantum dots for cell labeling.
  • Labeled primary cultured MSCs and evaluated fluorescence retention and cell function.
  • Constructed a piggyBack transposon vector for stable EmGFP expression in MSCs.
  • Utilized a novel suction device for stabilizing tissue during in vivo imaging.

Main Results:

  • PAMAM dendrimer-modified quantum dots showed faster endosomal escape and sustained fluorescence in MSCs compared to unmodified quantum dots.
  • Labeled MSCs maintained fluorescence intensity after intravenous injection.
  • Established MSCs with long-term EmGFP expression for in vivo tracking.
  • Demonstrated feasibility of in vivo real-time imaging of cell dynamics in mice.

Conclusions:

  • PAMAM dendrimer-modified quantum dots are effective for long-term, non-disruptive fluorescent labeling of MSCs.
  • This labeling strategy facilitates in vivo tracking of cell dynamics, essential for cell therapy development.
  • The developed techniques support advanced visualization of cell trafficking and therapeutic efficacy.