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Updated: May 23, 2026

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
Published on: March 9, 2016
QF-PCR: application, overview and review of the literature
Kathy Mann1, Caroline Mackie Ogilvie
1Cytogenetics Department, GSTS Pathology, Guy's and St Thomas' Hospital, London, UK. kathy.mann@gsts.com
Abstract:
Quantitative fluorescent polymerase chain reaction has been in diagnostic use in the UK for over 10 years and has proved to be a cost-effective, robust and accurate rapid prenatal test for common aneuploidies. Specific advantages include detection of triploidy, mosaicism and maternal cell contamination. Its application at our centre is described, with developments including stand-alone testing and improvements in strategies for the preparation and testing of chorionic villus biopsies.
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