TOUCH-UP gradient amplification method
Related Experiment Video
Updated: May 23, 2026

Fabrication of Gradient Nanopattern by Thermal Nanoimprinting Technique and Screening of the Response of Human Endothelial Colony-forming Cells
Published on: July 1, 2018
F B Rowther1, H Kardooni, T Warr
1Brain Tumour UK Neuro-oncology Research Centre, Research Institute in Healthcare Sciences, School of Applied Sciences, University of Wolverhampton, Wolverhampton, United Kingdom. fbrowther@wlv.ac.uk
Abstract:
We report a unique amplification technique that works efficiently and specifically over a temperature range, rather than at one specific temperature, throughout the amplification process. As bisulfite-modified DNA is one of the difficult to amplify templates, we used this technique to amplify regions of promoter-associated CpG island for 11 genes using this template. This technique amplified specific products for every gene without requiring any optimization.
Related Concept Videos
Gradient and Del Operator
Differential Staining Technique
Simple Staining Technique
Design Example: Vintage Mixing Console
The specifications for the pre-amplifier were clear. It needed to amplify the audio signal by a factor of 10, have an input impedance above 10...
