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Updated: May 23, 2026

Quantitative 31P NMR Analysis of Lignins and Tannins
Published on: August 2, 2021
Terpenoidal constituents of Eucalyptus loxophleba ssp. lissophloia
Jasmeen Sidana1, Sukhvinder Singh, Sunil Kumar Arora
1Department of Natural Products, National Institute of Pharmaceutical Education and Research, Sector 67, SAS Nagar, Punjab, India.
Context:
Eucalyptus has been a source of a number of biologically active compounds. The anti-leishmanial activity of terpenoids from Eucalyptus loxophleba (Benth.) ssp. lissophloia (Myrtaceae) has not yet been investigated.
Objective:
Isolation of the terpenoidal constituents for evaluation of in vitro anti-leishmanial activity against the Leishmania donovani (Dd8 strain) promastigotes.
Materials And Methods:
The chloroform-methanol (8:2) extract of dried leaves of Eucalyptus loxophleba was used to isolate terpenoidal constituents employing solvent partitioning, column chromatography and preparative high performance liquid chromatography and characterized from spectral data. The anti-leishmanial activity of the isolated compounds was tested in vitro using an Alamar blue assay against a culture of L. donovani (Dd8 strain) promastigotes.
Results:
Two new naturally occurring triterpenes, named loxanic acid and 3-acetyl loxanic acid together with four known ursane triterpenoids and one bis-monoterpene glycoside, cuniloside B isolated from the leaves showed anti-leishmanial activity (IC(50) 133 to 235 μM) against the promastigotes of the tested strain.
Conclusion:
The terpenes isolated from the leaves of E. loxophleba showed moderate anti-leishmanial activity.
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