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Related Experiment Video

Updated: May 23, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Functional assembly of aptamer binding sites by single-molecule cut-and-paste.

Mathias Strackharn1, Stefan W Stahl, Elias M Puchner

  • 1Center for Nanoscience and Department of Physics, University of Munich, Amalienstrasse 54, 80799 Munich, Germany.

Nano Letters
|April 4, 2012
PubMed
Summary

Researchers achieved precise bottom-up assembly of functional molecular ensembles by joining split aptamer halves. This method creates robust, single-molecule binding sites, significantly enhancing fluorescence for nanotechnology applications.

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Area of Science:

  • Nanotechnology
  • Molecular Biology
  • Biomolecular Engineering

Background:

  • Bottom-up assembly of functional molecular ensembles is a key goal in nanotechnology.
  • Novel properties emerge from the precise composition and arrangement of molecular constituents.
  • Developing methods for controlled, molecule-by-molecule assembly is crucial.

Purpose of the Study:

  • To demonstrate a novel single-molecule cut-and-paste technique for assembling functional molecular ensembles.
  • To create specific binding sites for malachite green using split aptamer halves.
  • To investigate the impact of assembly precision on binding site function and fluorescence properties.

Main Methods:

  • Utilized a single-molecule cut-and-paste approach to assemble split aptamer halves.

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Related Experiment Videos

Last Updated: May 23, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

  • Constructed molecule-by-molecule binding sites for the fluorophore malachite green.
  • Quantified fluorescence enhancement resulting from the reconstituted binding sites.
  • Assembled a micrometer-sized structure composed of over 500 reconstituted binding sites.

    • Main Results:

    • Achieved precise assembly of malachite green binding sites from split aptamer halves.
    • Demonstrated that only perfectly joined binding sites effectively immobilize the fluorophore.
    • Observed a fluorescence quantum yield enhancement of several orders of magnitude.
    • Successfully produced a large-scale (micrometer-sized) structure with over 500 functional binding sites.

    Conclusions:

    • This study presents the first demonstration of one-by-one, bottom-up functional biomolecular assembly.
    • The single-molecule cut-and-paste method offers a robust approach for creating functional molecular ensembles.
    • Precise assembly is critical for achieving desired properties, such as enhanced fluorescence, in nanomaterials.