Related Experiment Videos

Quercetin suppresses NF-κB and MCP-1 expression in a high glucose-induced human mesangial cell proliferation model

Pin Chen1, Qiyang Shi, Xiangjin Xu

  • 1Fuzhou General Hospital of Nanjing Military Command, Fuzhou, Fujian 350025, P.R. China.

International Journal of Molecular Medicine
|April 4, 2012
PubMed

Insights

Quercetin protects against diabetic nephropathy (DN) by inhibiting high glucose-induced human mesangial cell proliferation. Its protective effects are linked to the suppression of NF-κB signaling pathways.

Area of Science:

  • Nephrology
  • Cell Biology
  • Biochemistry

Background:

  • Diabetic nephropathy (DN) is a common diabetes complication characterized by mesangial cell proliferation.
  • The protective mechanisms of quercetin against DN remain unclear.

Purpose of the Study:

  • To elucidate the underlying mechanism of quercetin's protective effects against diabetic nephropathy.
  • To investigate quercetin's impact on high glucose-induced human mesangial cell (HMC) proliferation and associated molecular pathways.

Main Methods:

  • An in vitro model using high glucose (HG)-induced HMC proliferation.
  • Assessment of cell proliferation, cell cycle, and expression of NF-κB and MCP-1.
  • Utilized MTT assay, DNA staining, immunocytochemistry, and western blot analysis.

Main Results:

  • High glucose significantly increased HMC proliferation, G1 phase accumulation, and upregulated NF-κB and MCP-1 expression.
  • Quercetin treatment dose-dependently reversed these high glucose-induced effects.
  • Inhibition of NF-κB activation with PDTC suggested quercetin's effects are partially mediated by NF-κB signaling.

Conclusions:

  • Quercetin demonstrates protective effects against high glucose-induced mesangial cell proliferation.
  • These protective effects are, at least in part, mediated through the suppression of NF-κB signaling.

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...