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Targeted Metabolomics on Rare Primary Cells
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Targeted Metabolomics on Rare Primary Cells

Published on: February 23, 2024

Targeted metabolomics.

Lee D Roberts1, Amanda L Souza, Robert E Gerszten

  • 1Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Current Protocols in Molecular Biology
|April 4, 2012
PubMed
Summary
This summary is machine-generated.

This study details targeted metabolomics methods for analyzing blood plasma metabolites, including amino acids, lipids, and TCA cycle metabolites. These protocols utilize liquid chromatography-mass spectrometry for precise quantification in various physiological states.

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Last Updated: May 23, 2026

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Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Systems Biology

Background:

  • The metabolome represents the complete set of small molecules in biological systems, reflecting genomic and environmental influences.
  • Metabolomics provides insights into physiological states and diseases by quantifying metabolites.
  • Targeted metabolomics offers a focused approach to measure specific, known metabolites, complementing untargeted strategies.

Purpose of the Study:

  • To outline procedures for targeted metabolomics experiments.
  • To highlight the advantages of targeted metabolomics for metabolite analysis.
  • To describe protocols for extracting and analyzing key metabolites from blood plasma.

Main Methods:

  • Focus on targeted metabolomics methodologies.
  • Describes protocols for extracting nitrogenous metabolites (amino acids), lipids, and intermediary metabolites (TCA cycle oxoacids) from blood plasma.
  • Utilizes liquid chromatography-mass spectrometry (LC-MS) for targeted metabolite analysis.

Main Results:

  • Provides detailed procedures for targeted metabolomics experiments.
  • Demonstrates the extraction and analysis of diverse metabolite classes from blood plasma.
  • Highlights the utility of LC-MS for precise metabolite quantification.

Conclusions:

  • Targeted metabolomics provides a robust framework for analyzing specific metabolites in biological samples.
  • The described protocols enable comprehensive analysis of key metabolites in blood plasma.
  • This approach is valuable for understanding metabolic changes in health and disease.