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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
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Fountain flow cytometry.

Paul Johnson1

  • 1SoftRay, Inc, Laramie, Wisconsin, USA.

Current Protocols in Cytometry
|April 4, 2012
PubMed
Summary
This summary is machine-generated.

Fountain Flow Cytometry (FFC) offers a simple, low-cost method for detecting low concentrations of particles using LED-induced fluorescence and digital imaging. This technique excels in transparent fluids and challenging environments, enabling broad applications in cell and organism enumeration.

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Area of Science:

  • Biotechnology
  • Microscopy
  • Analytical Chemistry

Background:

  • Conventional flow cytometry is limited by particle concentration and fluorescence intensity.
  • Existing methods often require filtration and can struggle with high photometric backgrounds.

Purpose of the Study:

  • To present the physics behind Fountain Flow Cytometry (FFC), a novel hybrid technique.
  • To demonstrate FFC's capability for detecting and enumerating particles in diverse fluid samples.

Main Methods:

  • FFC combines flow cytometry principles with video epifluorescence microscopy.
  • Utilizes LED-induced fluorescence of labeled particles, eliminating filtration.
  • Employs a digital camera for fluorescence measurement as samples flow along the optical axis.
  • Automated particle counting using the Biocount computer program.

Main Results:

  • FFC successfully detects and enumerates particles in transparent and translucent fluids.
  • The method is effective in the presence of high photometric background, including unbound dye.
  • Demonstrated applicability for unwashed cells and a wide range of particle sizes (µm to mm).

Conclusions:

  • FFC is a versatile, cost-effective technology for low-concentration particle detection.
  • Its adaptability to various fluid types and challenging conditions expands its utility.
  • FFC shows promise for applications ranging from environmental monitoring to biological sample analysis.