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Neuronal profilin isoforms are addressed by different signalling pathways.

Kai Murk1, Nina Wittenmayer, Kristin Michaelsen-Preusse

  • 1Cellular Neurobiology, Zoological Institute, TU Braunschweig, Braunschweig, Germany.

Plos One
|April 4, 2012
PubMed
Summary
This summary is machine-generated.

Two profilin isoforms, PFN1 and PFN2a, are found in the central nervous system and their levels change with neuronal activity, suggesting distinct roles in synaptic plasticity and neuronal nuclei.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Profilins regulate actin dynamics, crucial for cell structure and function.
  • While most cells have one profilin, the central nervous system (CNS) has two: PFN1 and PFN2a.

Purpose of the Study:

  • To investigate the roles of PFN1 and PFN2a in neuronal activity-dependent structural plasticity.
  • To determine if the presence of two profilin isoforms is linked to neuronal complexity and activity.

Main Methods:

  • Immunoelectron microscopy and immunofluorescence on rodent neurons and brain sections.
  • Analysis of profilin levels in response to neuronal stimulation (KCl, BDNF) and NMDA receptor inhibition (APV).
  • Simultaneous labeling with isoform-specific antibodies to identify profilins in synapses and neuronal nuclei.

Main Results:

  • Both PFN1 and PFN2a are present in synapses, with higher abundance postsynaptically.
  • Neuronal stimulation increased synaptic levels of both PFN1 and PFN2a.
  • NMDA receptor inhibition decreased PFN2a but not PFN1; BDNF increased both isoforms.
  • Both profilins were found in neuronal nuclei, with activity-dependent changes in their levels.

Conclusions:

  • PFN1 and PFN2a have isoform-specific roles in actin dynamics within different signaling pathways at excitatory and inhibitory synapses.
  • Both profilins likely play functional roles in neuronal nuclei.
  • The findings support the hypothesis linking profilin isoform expression to neuronal activity and structural plasticity.