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Related Experiment Video

Updated: May 23, 2026

Disruption of the Mouse Blood-Brain Barrier by Small Extracellular Vesicles from Hypoxic Human Placentas
05:31

Disruption of the Mouse Blood-Brain Barrier by Small Extracellular Vesicles from Hypoxic Human Placentas

Published on: January 26, 2024

Microarray screening for novel preeclampsia biomarker candidates.

Olav Lapaire1, Simon Grill, Sebastien Lalevee

  • 1Department of Obstetrics and Gynecology, University Hospital Basel, Basel, Switzerland. olapaire@uhbs.ch

Fetal Diagnosis and Therapy
|April 5, 2012
PubMed
Summary
This summary is machine-generated.

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Researchers identified 14 potential preeclampsia biomarkers by analyzing placental gene expression. Four candidates were validated using RT-PCR, and two protein biomarkers, LHB and β-hCG, showed significant differences in preeclampsia patients.

Area of Science:

  • Obstetrics and Gynecology
  • Genomics and Molecular Biology
  • Biomarker Discovery

Background:

  • Preeclampsia diagnosis lacks reliable near-term prediction biomarkers.
  • Genome-wide gene expression screening in placental tissue offers a novel approach.
  • Severe preeclampsia cases were compared to healthy pregnancies.

Purpose of the Study:

  • To identify novel gene expression biomarkers for preeclampsia prediction.
  • To screen placental villous tissue for differentially expressed genes.
  • To validate potential biomarkers in maternal circulation.

Main Methods:

  • Gene expression profiling using Affymetrix arrays on pooled placental villous tissue.
  • Quantitative real-time RT-PCR for validating selected gene candidates.

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Last Updated: May 23, 2026

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  • Maternal plasma protein level analysis for biomarker confirmation.

    • Main Results:

    • Identified 896 significantly differentially expressed genes (p ≤ 0.05).
    • Selected 14 candidate genes (9 upregulated, 5 downregulated) present in circulation.
    • Validated four genes (β-hCG, HTRA4, LHB1, NOX4) via RT-PCR and confirmed LHB and β-hCG protein levels in maternal plasma.

    Conclusions:

    • Identified 14 potential novel biomarker candidates for preeclampsia.
    • Validated four candidates through RT-PCR and two via serum protein analysis.
    • Further research is needed to determine optimal marker combinations for imminent preeclampsia prediction.