Polylysine copolymers for gene delivery

Polylysine and its copolymers have been extensively used as nonviral polymeric gene carriers. Although polylysine on its own is toxic to cells, when polyethylene glycol is covalently linked to polylysine, toxicity is reduced and DNA transfection efficiency is increased. A degradable polylysine analog, polyaminobutyl glycolic acid, has been synthesized. Stearyl polylysine shows strong hydrophobic interactions with low-density lipoprotein and these components can be combined with DNA to form a "terplex" system that allows delivery of DNA to targeted cells and significant levels of transfection both in vitro and in vivo.