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Updated: May 23, 2026

In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
Published on: November 18, 2022
Influence of cheese matrix on lipid digestion in a simulated gastro-intestinal environment
Sophie Lamothe1, Marie-Michelle Corbeil, Sylvie L Turgeon
1Food Research and Development Centre, Agriculture and Agri-Food Canada, 3600 Casavant Boulevard West, Saint-Hyacinthe, Quebec, Canada J2S 8E3.
Abstract:
Foods are complex nutrient assemblies which are subjected to various industrial processes that can influence their nutritional value. The food matrix acts as a nutrient-release regulator, and further understanding of its behavior during digestion is essential. The objective of this study was to compare the kinetics of matrix degradation and fatty acids release for different cheeses in a gastro-intestinal environment. The relationship between the physical characteristics of the cheeses (rheological properties, microstructure) and their digestion pattern was also studied. Rheological measurements, compositional and microstructure analyses were performed on mild Cheddar, aged Cheddar, light Cheddar and Mozzarella cheeses. Cheese samples were subjected to simulated digestion. Matrix degradation index, free oil, free fatty acids and fat droplet distribution were analyzed after 5, 30, 60, 120, 150, 180 and 240 min of digestion. Mozzarella cheese showed the highest rate of matrix degradation, free oil and fatty acids release. Aged Cheddar cheese showed rapid degradation during the gastric phase, but was more resistant to the duodenal environment. Light Cheddar showed the opposite behavior, being highly resistant to the gastric environment; however, it underwent extensive degradation at the end of the duodenal phase. The extent of matrix degradation for mild Cheddar was similar to that of Mozzarella in the gastric phase but was much lower than that of other cheeses in the duodenal phase. The cheeses under study exhibited very different digestion patterns, and these differences are discussed in relation to cheese matrix composition, microstructure and rheological properties. Results suggest that cheese degradation and kinetics of fatty acids release are mainly driven by cheese physical characteristics.
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