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Related Concept Videos

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Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
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Related Experiment Video

Updated: May 23, 2026

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
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TOPLESS co-repressor interactions and their evolutionary conservation in plants.

Barry Causier1, James Lloyd, Laura Stevens

  • 1Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, UK.

Plant Signaling & Behavior
|April 6, 2012
PubMed
Summary

The TPL/TPR proteins in plants act as general transcriptional co-repressors. These repressors are involved in auxin signaling, a role conserved from mosses to flowering plants.

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Area of Science:

  • Plant molecular biology
  • Plant development
  • Transcriptional regulation

Background:

  • Arabidopsis TPL/TPR proteins are transcriptional co-repressors involved in diverse developmental processes.
  • They primarily interact with transcription factors containing repression domains (RDs).
  • RD sequences are diverse, yet TPL/TPRs bind to all known motifs, suggesting a general repressor role.

Purpose of the Study:

  • To investigate the role of TPL/TPR proteins in auxin signaling.
  • To examine interactions between moss Physcomitrella patens TPL/TPR proteins and auxin signaling components.
  • To determine if TPL's involvement in auxin signaling is evolutionarily conserved.

Main Methods:

  • Protein-protein interaction studies.
  • Comparative genomics analysis of TPL/TPR proteins in Physcomitrella patens and Arabidopsis.
  • Examination of interactions with auxin signaling pathway components (AUX/IAA and ARF proteins).

Main Results:

  • Moss TPL proteins interact with AUX/IAA and ARF proteins, similar to Arabidopsis.
  • These interactions suggest TPL/TPRs function in both forms of ARF-mediated transcriptional repression.
  • Evidence supports a conserved role for TPL in auxin signaling across plant evolution.

Conclusions:

  • TPL/TPR proteins function as general repressors of gene transcription in plants.
  • The involvement of TPL in auxin signaling is conserved between mosses and angiosperms, dating back approximately 450 million years.
  • This conservation highlights the fundamental role of TPL in plant development and signaling pathways.