Related Experiment Video

Updated: May 23, 2026

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
05:18

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Published on: June 9, 2020

E2F1 induces p19INK4d, a protein involved in the DNA damage response, following UV irradiation

Abel L Carcagno1, Luciana E Giono, Mariela C Marazita

  • 1Laboratorio de Biología Molecular, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria Pabellón II Piso 4, 1428, Ciudad de Buenos Aires, Argentina. abelc@qb.fcen.uba.ar

Molecular and Cellular Biochemistry
|April 6, 2012
PubMed

Insights

The p19INK4d protein, crucial for DNA repair, is upregulated by UV radiation via a transcriptional mechanism involving E2F1. This regulation is vital for cellular response to DNA damage.

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Genomics

Background:

  • The cellular DNA damage response is critical for maintaining genomic integrity.
  • p19INK4d, an INK4 family CDK inhibitor, has a dual role in DNA damage response, inhibiting proliferation and promoting repair.
  • p19INK4d is known to be upregulated by genotoxic agents, including UV irradiation.

Purpose of the Study:

  • To elucidate the transcriptional mechanism underlying p19INK4d induction following UV irradiation.
  • To investigate the role of E2F1 in the UV-induced upregulation of p19INK4d.
  • To demonstrate the physiological relevance of this regulatory mechanism in response to DNA damage.

Main Methods:

  • Analysis of p19INK4d transcriptional induction across different cell cycle phases.
  • Assessment of p19INK4d induction following UV treatment in E2F1-ablated cells and with DNA-binding inhibition.
  • Use of triplex-forming oligonucleotides to inhibit E2F response elements in the p19INK4d promoter.

Main Results:

  • p19INK4d induction by UV occurs transcriptionally during all cell cycle phases except mitosis.
  • E2F1 protein is essential for UV-induced p19INK4d upregulation, as demonstrated by ablation and DNA-binding inhibition experiments.
  • Inhibition of E2F regulation of the p19INK4d promoter blocks upregulation and sensitizes cells to DNA damage.

Conclusions:

  • This study describes the first mechanism for p19INK4d induction in response to UV irradiation.
  • E2F1 plays a critical role in the transcriptional activation of p19INK4d following UV-induced DNA damage.
  • The regulation of p19INK4d by E2F1 is physiologically relevant for the cellular response to DNA damage.

More Related Videos

Visualization of DNA Repair Proteins Interaction by Immunofluorescence07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

The Lambda Select cII Mutation Detection System07:08

The Lambda Select cII Mutation Detection System

Published on: April 26, 2018

Related Concept Videos

Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Mutations01:35

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...